![]() Though there are many laboratories that continue to use natural protein ladders, most now use recombinant standards. ![]() The broad, less distinct, bands make them less attractive for molecular weight estimation. Prestained natural proteins may produce broader bands than recombinant proteins, particularly when the proteins are prestained with more than one color. Recombinant Proteins: There is variability for prestained naturally occurring proteins, in the amount and location of dye that binds to each protein. These marker proteins were unstained, and were generally visualized on SDS-PAGE gels by staining the gel with Coomassie Brilliant Blue R250, or after western transfer by a stain such as Ponceau S. As a convenience, all ladders are supplied in gel loading buffer and are ready to use without heating or boiling.Originally molecular weight markers were a mixture of easy-to-purify proteins of known molecular weight. A broad range Spectra Protein Ladder is also available for more general applications.Īll Thermo Scientific Pierce Protein Molecular Weight Ladders come in a variety of package sizes. ![]() The Spectra™ High Range Protein Ladder contains proteins from 40 to 300 kilodaltons, making it ideal for analysis of large proteins, whereas the Spectra Low Range contains proteins from 1.7 to 40 kilodaltons for small protein research. Lastly, the pre-stained Thermo Scientific Spectra Multicolor Protein Ladders are available in three different size ranges for more precise analysis. All other proteins in both ladders carry a blue chromophore. The PageRuler Plus Pre-stained Ladder consists of nine proteins from 10 to 250 kilodaltons, with two orange reference bands and one green band. The PageRuler Pre-stained Ladder contains 10 proteins, ranging from 10 to 170 kilodaltons, and two different colored bands for reference. The tri-color PageRuler and PageRuler Plus Pre-stained Proteins Ladders offer a further level of convenience. The proteins in all ladders also carry the Strep-Tag II sequence for direct detection on Western blots using a Strep-tag detection system. All PageRuler Unstained Ladders contain at least one protein band of greater intensity for reference. Lastly, the PageRuler Low Range Unstained Ladder provides a mixture of seven proteins from 5 to 100 kilodaltons. The PageRuler Broad Range Unstained Protein Ladder contains 11 proteins from 5 to 250 kilodaltons. The standard PageRuler™ Unstained Protein Ladders provide a mixture of 14 proteins, ranging from 10 to 200 kilodaltons. The Thermo Scientific PageRuler Unstained Protein Ladders offer the user a choice of three molecular weight ranges for more accurate determinations and can be detected with coomassie or silver stains. The Pierce Unstained Protein Molecular Weight Ladders contain six proteins from 20 to 120 kilodaltons and can be used with coomassie-based and silver stains. The Pierce® Pre-stained Protein Molecular Weight Ladders contain seven single-color proteins, varying in size from 14.4 to 116 kilodaltons. The Thermo Scientific Pierce Protein Molecular Weight Ladders, the most economical option within the product family, are available pre-stained or unstained. All markers are made with Fermentas technology, which was acquired by the Thermo Fisher Scientific in the summer of 2010. These standards enable researchers to determine approximate molecular weight of proteins and verify Western blot transfer efficiency. Thermo Fisher Scientific, has introduced a family of protein molecular weight standards for use in monitoring protein separation during SDS polyacrylamide gel electrophoresis. ![]()
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